What Is Methylene Blue?


Welcome to my blog “What Is Methylene Blue?”.

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What Is Methylene Blue (MB)?

Methylene blue (MB) is considered to be the first synthetic medication ever used in humans (1).

It is an FDA-approved medication which has been used as an effective agent in malaria treatment, methemoglobinemia, and cyanide poisoning (8)


Methylene Blue And Alzheimer’s disease

Methylene Blue is currently being studied as potential therapy for mild cognitive impairment Alzheimer’s and Parkinson’sdisease, and other neurodegenerative disorders. All sharing a common problem with mitochondrial function.

While low-dose MB and low-level near-infrared light may produce different pleiotropic cellular effects, both interventions cause a similar up-regulation of mitochondrial respiration with similar benefits to protect nerve cells against degeneration (7)

Alzheimer’s disease is linked to an accumulation of the protein tau. MB has been shown in clinical trials to inhibit tau formation. It is being considered a drug for the treatment of Alzheimer’s disease. This is likely due to improving electron flow, ATP production, absorbing more external photon (light) absorption, and preventing excessive free radical damage or biophoton emission from stressed cells.

Methylene Blue supports brain cell respiration by raising oxygen levels throughout the body. As well as providing electrons to the electron transport chain in the mitochondria. The exact process is used to generate mitochondrial ATP when you eat. Methylene Blue, therefore, supplies this energy without the need to eat and essentially becomes more like a breatharian utilizing the power of oxygen (paramagnetic) and light by making more water.

MB has been tested in a human clinical trial in which patients with mild to moderate AD showed both cognitive and cerebral blood flow improvements after MB treatmen

Methylene Blue And Mitochondrial Function

Low-dose MB stimulates mitochondrial respiration by donating electrons to the electron transport chain.

Its role in the mitochondria has elicited much of its renewed interest in recent years. MB can reroute electrons in the mitochondrial electron transfer chain directly from NADH to cytochrome c, increasing the activity of complex IV and effectively promoting mitochondrial activity while mitigating oxidative stress. Mitochondrial dysfunction has been identified as a seemingly unifying pathological phenomenon across a wide range of neurodegenerative disorders, which thus positions methylene blue as a promising therapeutic.

Methylene Blue And Depression

A low dose of MB has an antidepressant effect because it acts as a monoamine oxidase (MAO) inhibitor. Monoamine oxidases are enzymes that remove the neurotransmitters norepinephrine, serotonin, and dopamine from the brain. Inhibition of MAO prevents monoamine neurotransmitters’ breakdown, which directly leads to an increased level of these neurotransmitters.

Methylene Blue As a Nootropic

As a nootropic, Methylene Blue is used to enhance mitochondrial function, increase cerebral blood flow, and acts as an antidepressant.

Methylene Blue And COVID-19

This molecule was found to inhibit the interaction of COVID19 virus and target cells in dose dependent manner. It was also found to inhibit interaction of viron with host cells, by inhibiting interaction of SARS CoV2 spike protein and ACE inhibitor receptor interactions. (3)

Methylene Blue And Thyroid Health

Surprisingly, blood thyroxine levels increased consistently in rats after MB treatment (4)

MB in an animal study was shown to increase T4 levels of thyroxine which might be indirectly connected to the same animal study review of decreased prolactin levels after stimulating a stress response with the administration of estrogen.

Methylene Blue And Viral Infections

Low MB doses combined with specific light spectra (especially UV) can effectively combat viruses.

Methylene Blue Is An Antimicrobial

MB has unique gut healing abilities by displacing candida infections in hyphal conditions and transitions, which, if not resolved, could lead to colonization and severe health issues.

MB does support not only fungal issues but also bacterial infections leading to irradiation alongside other treatments and combinations such as nanoparticle silver or phototherapy.

MB And Skin Health

Methylene Blue at a low dose was shown to stimulate improvements and increased protein activation in collagen and elastin production.

MB And Bipolar Disorder

In psychiatry, methylene blue has been used for over a century. It was tried successfully in the treatment of psychotic and mood disorders and as a memory enhancer in fear-extinction training. Particularly promising results have been obtained in both short- and long-term treatment of bipolar disorder. In these studies, MB produced an antidepressant and anxiolytic effect without risk of a switch into mania. Long-term use of methylene blue in bipolar disorder led to a better stabilization and a reduction in residual symptoms of the illness. It is usually well tolerated, but caution is needed in the light of its inhibitory effect on monoamine oxidase A. (5)

MB And Cancer

There is accumulating evidence providing a proof of concept that enhancement of mitochondrial oxidative phosphorylation via alternative mitochondrial electron transfer may offer protective action against neurodegenerative diseases and inhibit cancers proliferation (6)

MB Improves Memory

Animal studies have shown that a single low dose of MB enhances long-term contextual memory.

When should I Take MB?

MB should be taken in the morning to compliment the natural morning dominant red light spectrum. There is no hard rule to follow but from my personal experience, experience with clients, and speaking with Dr. Scott Sherr morning and mid-morning times are preferential.

Methylene Blue Recommended Dosage

There is no true recommended dose for MB. David Tomen from Nootropic Expert recommends  starting with the lowest dose of 0.5 mg/kg and see how you react.

He also explains how MB is water-soluble so you don’t need to take it with a meal, or healthy fat like some nootropics.


  1. From Mitochondrial Function to Neuroprotection-an Emerging Role for MB (click here)
  2. The Potentials of MB as an Anti-Aging Drug (click here)
  3. MB in management of COVID19 (click here)
  4. MB as an endocrine modulator: interactions with thyroid hormones (click here)
  5. MB in the Treatment of Neuropsychiatric Disorders (click here)
  6. Alternative mitochondrial electron transfer for the treatment of neurodegenerative diseases and cancers: MB connects the dots (click here)
  7. Protection against neurodegeneration with low-dose MB and near-infrared light (click here)
  8. Mitochondria as a target for neuroprotection: role of MB and photobiomodulation (click here)
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